Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S134

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Fusion protein amino acids 1724-1956 of rat Nav1.8

Localization

Membrane

Specificity

Detects approx 220kDa. No cross reactivity against other Nav channels.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Nav1.8 Antibody (S134) - BSA Free

Western Blot: Nav1.8 Antibody (S134) [NBP1-47615]

Western Blot: Nav1.8 Antibody (S134) [NBP1-47615]

Western Blot: Nav1.8 Antibody (S134) [NBP1-47615] - analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Nav1.8 Antibody (S134) [NBP1-47615]

Immunocytochemistry/ Immunofluorescence: Nav1.8 Antibody (S134) [NBP1-47615]

Immunocytochemistry/Immunofluorescence: Nav1.8 Antibody (S134) [NBP1-47615] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134 (NBP1-47615). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (NBP1-47615) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.8 Antibody (D) Composite.
Immunohistochemistry: Nav1.8 Antibody (S134) [NBP1-47615]

Immunohistochemistry: Nav1.8 Antibody (S134) [NBP1-47615]

Immunohistochemistry: Nav1.8 Antibody (S134) [NBP1-47615] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
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Applications for Nav1.8 Antibody (S134) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of Nav1.8 Antibody was sufficient for detection of Nav1.8 in 10 ug of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nav1.8

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).

Long Name

Sodium channel protein type 10 subunit alpha

Alternate Names

hPN3, PN3, SCN10A

Entrez Gene IDs

6336 (Human); 20264 (Mouse); 29571 (Rat)

Gene Symbol

SCN10A

UniProt

Q62968 (Rat)

Additional Nav1.8 Products

Product Documents for Nav1.8 Antibody (S134) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nav1.8 Antibody (S134) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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