NCALD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15037

Novus Biologicals, part of Bio-Techne
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NBP2-15037
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Chicken (99%), Porcine (100%), Rhesus Macaque (100%), Xenopus (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCALD Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCALD. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCALD Antibody

Western Blot: NCALD Antibody [NBP2-15037]

Western Blot: NCALD Antibody [NBP2-15037]

Western Blot: NCALD Antibody [NBP2-15037] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037]

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037]

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037] - Rat brain. Neurocalcin delta antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037]

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037]

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037] - Mouse brain. Neurocalcin delta antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for NCALD Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCALD

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

MGC33870, MGC74858, neurocalcin delta, neurocalcin-delta

Gene Symbol

NCALD

UniProt

P61601 (Human)

Additional NCALD Products

Product Documents for NCALD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCALD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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