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NCF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33502

Novus Biologicals, part of Bio-Techne
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NBP2-33502
NBP2-33502-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: YMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENHQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCF1 Antibody

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human spleen shows high expression.
Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human bone marrow shows moderate cytoplasmic positivity in a subset of hematopoietic cells.
Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502]

Immunohistochemistry-Paraffin: NCF1 Antibody [NBP2-33502] - Staining of human cerebral cortex shows low expression as expected.

Applications for NCF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCF1

NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

Alternate Names

47 kDa neutrophil oxidase factor, FLJ79451, NADPH oxidase organizer 2, NCF-1, NCF1A, neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein, neutrophil cytosolic factor 1, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1), neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2NCF-47K, Nox-organizing protein 2, p47phox, SH3 and PX domain-containing protein 1A, SH3PXD1Ap47-phox

Gene Symbol

NCF1

UniProt

Additional NCF1 Products

Product Documents for NCF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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