NCK1 Antibody (4R1N9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16759
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4R1N9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NCK1 Antibody (4R1N9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333). PFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCK1 Antibody (4R1N9)
![Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759]](https://resources.bio-techne.com/images/products/NCK1-Antibody-4R1N9-Western-Blot-NBP3-16759-img0003.jpg "Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759]")
Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759]
Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759]](https://resources.bio-techne.com/images/products/NCK1-Antibody-4R1N9-Immunocytochemistry-Immunofluorescence-NBP3-16759-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759]")
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759]
Immunocytochemistry/Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759]](https://resources.bio-techne.com/images/products/NCK1-Antibody-4R1N9-Immunohistochemistry-Paraffin-NBP3-16759-img0001.jpg "Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759]")
Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759]
Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for NCK1 Antibody (4R1N9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NCK1
Long Name
Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1
Alternate Names
cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha
Gene Symbol
NCK1
Additional NCK1 Products
Product Documents for NCK1 Antibody (4R1N9)
Product Specific Notices for NCK1 Antibody (4R1N9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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