Skip to main content

NCK2 Antibody (S01-3C6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15063

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-15063-100ul
NBP3-15063-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-3C6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for NCK2 Antibody (S01-3C6)

Immunogen

A synthetic peptide of human NCK2 (Uniprot#: O43639)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCK2 Antibody (S01-3C6)

Western Blot: NCK2 Antibody (S01-3C6) [NBP3-15063]

Western Blot: NCK2 Antibody (S01-3C6) [NBP3-15063]

Western Blot: NCK2 Antibody (S01-3C6) [NBP3-15063] - Western blot detection of NCK2 in K562, Rat Brain, C6, 3T3, Hela cell lysates using NCK2 Rabbit mAb (1:1000 diluted). Predicted band size: 43kDa. Observed band size: 43kDa.
Immunocytochemistry/ Immunofluorescence: NCK2 Antibody (S01-3C6) [NBP3-15063]

Immunocytochemistry/ Immunofluorescence: NCK2 Antibody (S01-3C6) [NBP3-15063]

Immunocytochemistry/Immunofluorescence: NCK2 Antibody (S01-3C6) [NBP3-15063] - Immunofluorescence of NCK2 (green) in Hela cells using NCK2 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for NCK2 Antibody (S01-3C6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCK2

NCK2 encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Alternate Names

GRB4, NCK adaptor protein 2cytoplasmic protein NCK2, Nck-2, NCKbeta, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK-beta

Gene Symbol

NCK2

Additional NCK2 Products

Product Documents for NCK2 Antibody (S01-3C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCK2 Antibody (S01-3C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...