Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NCOA2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 700 and 750 of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28552353).

Specificity

Antibody was affinity purified using an epitope specific to SRC2 immobilized on solid support.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA2 Antibody

Western Blot: NCOA2 Antibody [NB100-388]

Western Blot: NCOA2 Antibody [NB100-388]

Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NCOA2 Antibody [NB100-388]

Immunoprecipitation: NCOA2 Antibody [NB100-388]

Immunoprecipitation: NCOA2 Antibody [NB100-388] - Detection of human NCOA2/ SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRC2 was also immunoprecipitated by a previous lot (lot 1) and by rabbit anti-NCOA1/SRC2 antibody NBP2-22162, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for NCOA2 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
NB 100-388 may be used for Western Blot, where a band at ~160 kDa is seen, representing SRC2 protein. Though this antibody will recognize cytosolic protein in Western blot, it does much better with nuclear extracts. It may also be used for immunoprecipitation on HeLa nuclear extracts. Suggested working dilutions: * Western Blot - 1:1,000-1:10,000 Immunocytochemistry - Not Determined Immunoprecipitation - 1-4 ug/mg *The investigator should determine the optimal working dilution for a specific application. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28552353).

Reviewed Applications

Read 1 review rated 4 using NB100-388 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA2

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Long Name

Nuclear Receptor Co-activator 2

Alternate Names

bHLHe75, HTIF2, KAT13C, TIF2

Entrez Gene IDs

10499 (Human)

Gene Symbol

NCOA2

UniProt

Q15596 (Human)

Additional NCOA2 Products

Product Documents for NCOA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCOA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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