Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-224, NB500-160, NB200-347, NBP1-06527, NBP1-19102

Scientific Data Images for NCOA3/AIB1 Antibody

Western Blot: NCOA3/AIB1 Antibody [NB100-314]

Western Blot: NCOA3/AIB1 Antibody [NB100-314]

Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: NCOA3/AIB1 Antibody [NB100-314]

Western Blot: NCOA3/AIB1 Antibody [NB100-314]

Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.

Applications for NCOA3/AIB1 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
NB 100-314 may be used for Western blot and immunoprecipitation. This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be immunoprecipitated and analyzed on Western blot using nuclear or cytosolic extracts. Suggested working dilutions: * Western Blot - 1:1,000 ~ 1:10,000 (5 ug nuclear extract) Immunocytochemistry - Not Done Immunoprecipitation - 1-4 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.

Reviewed Applications

Read 1 review rated 3 using NB100-314 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA3

Steroid receptor coactivator 3 (SRC3) is a ~154 kDa nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in hormone-dependent fashion. SRC3 recruits 2 other nuclear factors, CBP and PCAF, and thus plays a central role in creating a multisubunit coactivator complex.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

8202 (Human)

Gene Symbol

NCOA3

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCOA3/AIB1 Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-224, NB500-160, NB200-347, NBP1-06527, NBP1-19102

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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