NCOA3/AIB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33720

Novus Biologicals, part of Bio-Techne
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NBP1-33720
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCOA3 Antibodies »

Product Summary for NCOA3/AIB1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NCOA3/AIB1. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA3/AIB1 Antibody

Western Blot: NCOA3/AIB1 Antibody [NBP1-33720]

Western Blot: NCOA3/AIB1 Antibody [NBP1-33720]

Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NCOA3 antibody [N1N2], N-term.
Immunocytochemistry/ Immunofluorescence: NCOA3/AIB1 Antibody [NBP1-33720]

Immunocytochemistry/ Immunofluorescence: NCOA3/AIB1 Antibody [NBP1-33720]

Immunocytochemistry/Immunofluorescence: NCOA3/AIB1 Antibody [NBP1-33720] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-33720]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-33720]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-33720] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
View 1 more images

Applications for NCOA3/AIB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCOA3

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Gene Symbol

NCOA3

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA3/AIB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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