Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all NCOA4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA4 Antibody - BSA Free

Immunohistochemistry: NCOA4 Antibody [NBP1-28681]

Immunohistochemistry: NCOA4 Antibody [NBP1-28681]

Immunohistochemistry: NCOA4 Antibody [NBP1-28681] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARA70 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681]

Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681]

Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: NCOA4 Antibody [NBP1-28681]

Immunoprecipitation: NCOA4 Antibody [NBP1-28681]

Immunoprecipitation: NCOA4 Antibody [NBP1-28681] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARA70 antibody NBP1-28681 used for IP at 10 ug/mg lysate. ARA70 was also immunoprecipitated by rabbit anti-ARA70 antibody NBP1-28720, which recognizes a downstream epitope. For blotting immunoprecipitated ARA70, NBP1-28720 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NCOA4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA4

ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.

Alternate Names

Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator

Entrez Gene IDs

8031 (Human)

Gene Symbol

NCOA4

UniProt

NP_005428.1 (Human)

Additional NCOA4 Products

Product Documents for NCOA4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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