Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NCOA6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2000 and the C-terminus (residue 2063) of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA6 Antibody

Western Blot: NCOA6 Antibody [NB200-336]

Western Blot: NCOA6 Antibody [NB200-336]

Western Blot: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-336 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: NCOA6 Antibody [NB200-336]

Immunoprecipitation: NCOA6 Antibody [NB200-336]

Immunoprecipitation: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot of Immunoprecipitates

Applications for NCOA6 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:10000
Application Notes
This antibody is useful for Western Blot and Immunoprecipitation. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA6

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Alternate Names

Activating signal cointegrator 2, AIB3NRC RAP250, Amplified in breast cancer protein 3, ASC-2, Cancer-amplified transcriptional coactivator ASC-2, KIAA0181PPAR-interacting protein, NRCamplified in breast cancer-3 protein, nuclear receptor coactivator 6, Nuclear receptor coactivator RAP250, Nuclear receptor-activating protein, 250 kDa, peroxisome proliferator-activated receptor interacting protein, PRIPPeroxisome proliferator-activated receptor-interacting protein, RAP250Thyroid hormone receptor-binding protein, thyroid hormone receptor binding protein, TRBPASC2activating signal cointegrator-2

Gene Symbol

NCOA6

UniProt

Q14686 (Human)

Additional NCOA6 Products

Product Documents for NCOA6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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