ND6 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94464
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 80-174 of human ND6 (YP_003024037.1). LIREDPIGAGALYDYGR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ND6 Antibody - Azide and BSA Free
Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]
Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.Immunohistochemistry-Paraffin: ND6 Antibody - Azide and BSA Free [NBP2-94464]
Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Rat kidney using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Western Blot: ND6 AntibodyAzide and BSA Free [NBP2-94464]
Western Blot: ND6 Antibody [NBP2-94464] - Analysis of HepG2, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.Applications for ND6 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:50 - 1:200
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ND6
Alternate Names
mitochondrially encoded NADH dehydrogenase 6, MTND6, MT-ND6, NADH dehydrogenase subunit 6, NADH dehydrogenase, subunit 6 (complex I), NADH6
Gene Symbol
ND6
Additional ND6 Products
Product Documents for ND6 Antibody - Azide and BSA Free
Product Specific Notices for ND6 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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