NDC80 Antibody (JE31-10)

Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419]

Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: HepG2 cell lysate
Lane 8: LO2 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.