NDC80 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1100

Novus Biologicals, part of Bio-Techne
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NB100-1100
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NDC80 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YEKKATLIKSSEE corresponding to C-Terminus according to NP_006092.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDC80 Antibody

Western Blot: NDC80 Antibody [NB100-1100]

Western Blot: NDC80 Antibody [NB100-1100]

Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100]

Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100]

Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100]

Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100]

Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NDC80 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 75-80 kDa band observed in Hela lysate (predicted MW of 74 kDa band according to NP_006092). IHC: Paraffin embedded Human Liver and Adrenal Gland.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDC80

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), hsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Entrez Gene IDs

10403 (Human)

Gene Symbol

NDC80

UniProt

NP_006092. (Human)

Additional NDC80 Products

Product Documents for NDC80 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDC80 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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