NDC80 [p Ser55] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13726

Novus Biologicals, part of Bio-Techne
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NBP3-13726
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Marsupial

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDC80 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human NDC80. The exact sequence is proprietary.

Reactivity Notes

Kangaroo rat

Modification

p Ser55

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for NDC80 [p Ser55] Antibody

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody
Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 (phospho Ser 55) antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726]

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)
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Applications for NDC80 [p Ser55] Antibody

Application
Recommended Usage

Immunoprecipitation

2-4 μg/mL for 1 mg cell extract

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDC80

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), hsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Gene Symbol

NDC80

Additional NDC80 Products

Product Documents for NDC80 [p Ser55] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDC80 [p Ser55] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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