NDP52 Antibody (OTI4H5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72897

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NDP52 Antibodies »

Product Summary for NDP52 Antibody (OTI4H5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDP52 Antibody (OTI4H5) - Azide and BSA Free

Western Blot: NDP52 Antibody (OTI4H5)Azide and BSA Free [NBP2-72897]

Western Blot: NDP52 Antibody (OTI4H5)Azide and BSA Free [NBP2-72897]

Western Blot: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.
Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897]

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897]

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.
Western Blot: NDP52 Antibody (OTI4H5)Azide and BSA Free [NBP2-72897]

Western Blot: NDP52 Antibody (OTI4H5)Azide and BSA Free [NBP2-72897]

Western Blot: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.
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Applications for NDP52 Antibody (OTI4H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDP52

NDP52 is encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Alternate Names

Antigen nuclear dot 52 kDa protein, calcium binding and coiled-coil domain 2, calcium-binding and coiled-coil domain-containing protein 2, NDP52MGC17318, Nuclear domain 10 protein 52, Nuclear domain 10 protein NDP52, Nuclear dot protein 52

Gene Symbol

CALCOCO2

Additional NDP52 Products

Product Documents for NDP52 Antibody (OTI4H5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NDP52 Antibody (OTI4H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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