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NDRG4 Antibody (2G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00065009-M01

Novus Biologicals, part of Bio-Techne
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H00065009-M01

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NDRG4 (AAH11795.1, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPECWDGEHDIETPYGLLHVVIRGSPKGNRPAILTYHDVGLNHKLCFNTFFNFEDMQEITKHFVVCHVDAPGQQVGASQFPQGYQFPSMEQLAAMLPSVVQHFGFKYVIGIGVGAGAYVLAKFALIFPDLVEGLVLVNIDPNGKGWIDWAATKLSGLTSTLPDTVLSHLFSQEELVNNTELVQSYRQQIGNVVNQANLQLFWNMYNSRRDLDINRPGTVPNAKTLRCPVMLVVGDNAPAEDGVVECNSKLDPTTTTFLKMADSGGLPQVTQPGKLTEAFKYFLQGMGYMPSASMTRLARSRTASLTSASSVDGSRPQACTHSESSEGLGQVNHTMEVSC

Localization

Cytoplasmic

Specificity

NDRG4 - NDRG family member 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NDRG4 Antibody (2G3)

Western Blot: NDRG4 Antibody (2G3) [H00065009-M01]

Western Blot: NDRG4 Antibody (2G3) [H00065009-M01]

Western Blot: NDRG4 Antibody (2G3) [H00065009-M01] - NDRG4 monoclonal antibody (M01), clone 2G3. Analysis of NDRG4 expression in Raw 264.7.
Immunohistochemistry-Paraffin: NDRG4 Antibody (2G3) [H00065009-M01]

Immunohistochemistry-Paraffin: NDRG4 Antibody (2G3) [H00065009-M01]

Immunohistochemistry-Paraffin: NDRG4 Antibody (2G3) [H00065009-M01] - Analysis of monoclonal antibody to NDRG4 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 3 ug/ml.
ELISA: NDRG4 Antibody (2G3) [H00065009-M01]

ELISA: NDRG4 Antibody (2G3) [H00065009-M01]

ELISA: NDRG4 Antibody (2G3) [H00065009-M01] - Detection limit for recombinant GST tagged NDRG4 is approximately 3ng/ml as a capture antibody.

Applications for NDRG4 Antibody (2G3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and Immunohistochemistry (Paraffin).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 17008871)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDRG4

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

BDM1, Brain development-related molecule 1, DKFZp686I1615, FLJ30586, KIAA1180FLJ42011, NDRG family member 4, protein NDRG4, SMAP-8MGC19632, smooth muscle-associated protein 8, Vascular smooth muscle cell-associated protein 8

Entrez Gene IDs

65009 (Human)

Gene Symbol

NDRG4

Additional NDRG4 Products

Product Documents for NDRG4 Antibody (2G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDRG4 Antibody (2G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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