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NDST3 Antibody (5B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009348-M01

Novus Biologicals, part of Bio-Techne
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H00009348-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NDST3 (NP_004775, 162 a.a. ~ 259 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IGFHKTSEKSVQSFQLKGFPFSIYGNLAVKDCCINPHSPLIRVTKSSKLEKGSLPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIH

Reactivity Notes

Human. Other species not tested.

Localization

Golgi apparatus membrane; Single-pass type II membrane protein.

Specificity

NDST3 - N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NDST3 Antibody (5B9)

Western Blot: NDST3 Antibody (5B9) [H00009348-M01]

Western Blot: NDST3 Antibody (5B9) [H00009348-M01]

Western Blot: NDST3 Antibody (5B9) [H00009348-M01] - NDST3 monoclonal antibody (M01), clone 5B9 Analysis of NDST3 expression in Hela S3 NE.
Sandwich ELISA: NDST3 Antibody (5B9) [H00009348-M01] - Detection limit for recombinant GST tagged NDST3 is approximately 1ng/ml as a capture antibody.

Applications for NDST3 Antibody (5B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDST3

NDST3 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3) is an essential monomeric enzyme with 2 catalytic activities. It catalyzes both the N-deacetylation and the N-sulfation of N-acetylglucosamine residues (GlcNAc) in heparan sulfate and heparin. NDST3 has high deacetylase activity but low sulfotransferase activity.

Alternate Names

bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3, EC 2.8.2.8, GlcNAc N-deacetylase/ N-sulfotransferase 3, Glucosaminyl N-deacetylase/N-sulfotransferase 3, hNDST-3, HSST3MGC130028, MGC130029, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3, NDST-3, N-heparan sulfate sulfotransferase 3, N-HSST 3

Entrez Gene IDs

9348 (Human)

Gene Symbol

NDST3

OMIM

603950 (Human)

Additional NDST3 Products

Product Documents for NDST3 Antibody (5B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDST3 Antibody (5B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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