NDUFA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29884

Novus Biologicals, part of Bio-Techne
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NBP3-29884
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFA5 Antibodies »

Product Specifications

Immunogen

Between 66 and 116

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFA5 Antibody

NDUFA5 Antibody

Immunoprecipitation:NDUFA5 AntibodyNBP3-29884] -

Immunoprecipitation:NDUFA5 AntibodyNBP3-29884] -Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody NBP3-29884 (lot NBP3-29884-1) used for IP at 6 µg per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody. For blotting immunoprecipitated NDUFA5, NBP3-29884 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for NDUFA5 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NDUFA5

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

Alternate Names

B13,5 (13kD, B13), DKFZp781K1356, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa, UQOR13FLJ12147

Gene Symbol

NDUFA5

Additional NDUFA5 Products

Product Documents for NDUFA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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