NDUFB8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05965
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human NDUFB8 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for NDUFB8 Antibody
Western Blot: NDUFB8 Antibody [NBP3-05965]
Western Blot: NDUFB8 Antibody [NBP3-05965] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDaImmunocytochemistry/ Immunofluorescence: NDUFB8 Antibody [NBP3-05965]
Immunocytochemistry/Immunofluorescence: NDUFB8 Antibody [NBP3-05965] - Staining of NDUFB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB8 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965]
Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965] - Staining of human NDUFB8 in human kidney with rabbit polyclonal antibody at 1:100 dilution.Applications for NDUFB8 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NDUFB8
Alternate Names
CI-ASHImitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa, NADH:ubiquinone oxidoreductase ASHI subunit, NADH-ubiquinone oxidoreductase ASHI subunit
Gene Symbol
NDUFB8
Additional NDUFB8 Products
Product Specific Notices for NDUFB8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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