NDUFC2 Antibody (JE65-31)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32637
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-31
Concentration
1 mg/ml
Product Summary for NDUFC2 Antibody (JE65-31)
Immunogen
Recombinant protein within human NDUFC2 aa 1-119. (Uniprot: O95298)
Localization
Mitochondrion inner membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NDUFC2 Antibody (JE65-31)
Western Blot: NDUFC2 Antibody (JE65-31) [NBP3-32637]
Western Blot: NDUFC2 Antibody (JE65-31) [NBP3-32637] - Western blot analysis of NDUFC2 on different lysates with Rabbit anti-NDUFC2 antibody (NBP3-32637) at 1/500 dilution.Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: A549 cell lysate
Lane 4: Human kidney tissue lysate (20 ug/Lane)
Lysates/proteins at 10 ug/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32637) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for NDUFC2 Antibody (JE65-31)
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NDUFC2
Alternate Names
B14.5b, CI-B14.5b, complex I subunit B14.5b, Complex I-B14.5b, HLC-1NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2 (14.5kD, B14.5b), Human lung cancer oncogene 1 protein, NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa, NADH dehydrogenase [ubiquinone] 1 subunit C2, NADHDH2, NADH-ubiquinone oxidoreductase subunit B14.5b
Gene Symbol
NDUFC2
Additional NDUFC2 Products
Product Documents for NDUFC2 Antibody (JE65-31)
Product Specific Notices for NDUFC2 Antibody (JE65-31)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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