NDUFS2 Antibody (PSH03-26)

Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639]

Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - NDUFS2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32639 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32639 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: NBP3-32639 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of NBP3-32639 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 43 seconds

Western Blot: NDUFS2 Antibody (PSH03-26) [NBP3-32639]

Western Blot: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Jurkat cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: A431 cell lysate (20 ug/Lane)
Lane 5: Raji cell lysate (20 ug/Lane)
Lane 6: SK-Br-3 cell lysate (20 ug/Lane)
Lane 7: K-562 cell lysate (20 ug/Lane)
Lane 8: NIH/3T3 cell lysate (20 ug/Lane)
Lane 9: RAW264.7 cell lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (20 ug/Lane)
Lane 11: Mouse brain tissue lysate (40 ug/Lane)
Lane 12: Mouse kidney tissue lysate (40 ug/Lane)
Lane 13: Rat brain tissue lysate (40 ug/Lane)
Lane 14: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 53 kDa
Observed band size: 45 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32639) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: NDUFS2 Antibody (PSH03-26) [NBP3-32639]

Immunohistochemistry: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.