Ndufs4 Antibody (1A1)

Novus Biologicals, part of Bio-Techne | Catalog # H00004724-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ndufs4 Antibodies »

Product Specifications

Immunogen

NDUFS4 (NP_002486, 66 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNMVLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSWNKRTRVSTK

Specificity

NDUFS4 - NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ndufs4 Antibody (1A1)

Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01]

Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01]

Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.Lane 1: NDUFS4 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01]

Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01]

Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01]

Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01]

Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi ( Cat # H00004724-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody (M01), clone 1A1 (Cat # H00004724-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for Ndufs4 Antibody (1A1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ndufs4

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes.

Alternate Names

AQDQ, AQDQmitochondrial, CI-18 kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 4 (18kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Qreductase), NADH-coenzyme Q reductase, 18-KD, NADH-ubiquinone oxidoreductase 18 kDa subunit

Entrez Gene IDs

4724 (Human)

Gene Symbol

NDUFS4

OMIM

252010 (Human)

UniProt

NP_002486 (Human)

Additional Ndufs4 Products

Product Documents for Ndufs4 Antibody (1A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ndufs4 Antibody (1A1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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