NDUFS6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49831

Novus Biologicals, part of Bio-Techne
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NBP1-49831
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NDUFS6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RIRFVGRQKEVNEN corresponding to internal region according to NP_004544.1.

Epitope

RIRFVGRQKEVNEN - Internal region

Reactivity Notes

Expected from sequence similarity: Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDUFS6 Antibody

Western Blot: NDUFS6 Antibody [NBP1-49831]

Western Blot: NDUFS6 Antibody [NBP1-49831]

Western Blot: NDUFS6 Antibody [NBP1-49831] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDUFS6 Antibody [NBP1-49831]

Immunohistochemistry-Paraffin: NDUFS6 Antibody [NBP1-49831]

Immunohistochemistry-Paraffin: NDUFS6 Antibody [NBP1-49831] - 5ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NDUFS6 Antibody [NBP1-49831]

Western Blot: NDUFS6 Antibody [NBP1-49831]

Western Blot: NDUFS6 Antibody [NBP1-49831] - Analysis of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NDUFS6 Antibody

Application
Recommended Usage

Immunohistochemistry

0.01-0.03ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1:100-1:2000
Application Notes
Western blot: Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Kidney.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFS6

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction is made up of 7 subunits, including NDUFS6. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI13KDA, Complex I-13kD-A, mitochondrial respiratory chain, 13-kD subunit, NADH dehydrogenase (ubiquinone) Fe-S protein 6 (13kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, NADH:ubiquinone oxidoreductase NDUFS6 subunit, NADH-ubiquinone oxidoreductase 13 kDa-A subunit

Entrez Gene IDs

4726 (Human); 407785 (Mouse); 29478 (Rat)

Gene Symbol

NDUFS6

UniProt

NP_004544.1 (Human)

Additional NDUFS6 Products

Product Documents for NDUFS6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDUFS6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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