NEBL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45223
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ELQRLKRTQE corresponding to internal region according to NP_998734.1, NP_001166955.1.
Epitope
internal region
Specificity
This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for NEBL Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10 - 1:500
Peptide ELISA
Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 30 kDa band in human brain (frontal cortex) lysates, but not in human, mouse and rat heart lysates after 1 ug/ml antibody staining. This protein has a calculated MW of 31.2 kDa band according to NP_998734.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P usage is reported in scientific literature (PMID: 24449343).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NEBL
Alternate Names
Actin-binding Z-disk protein, FLJ53769, LASP2, LIM-nebulette, LNEBL, MGC119746, MGC119747, nebulette
Gene Symbol
NEBL
UniProt
Additional NEBL Products
Product Documents for NEBL Antibody
Product Specific Notices for NEBL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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