Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F8

Concentration

0.54 mg/ml

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Product Specifications

Immunogen

Full length human recombinant protein of human PVRL1(NP_002846) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nectin-1/PVRL1 Antibody (OTI6F8)

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8) [NBP2-01198]

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8) [NBP2-01198]

Western Blot: PVRL1 Antibody (6F8) [NBP2-01198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1.
Immunocytochemistry/Immunofluorescence: PVRL1 Antibody (6F8) [NBP2-01198] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PVRL1.
Immunohistochemistry-Paraffin: Nectin-1/PVRL1 Antibody (OTI6F8) [NBP2-01198]

Immunohistochemistry-Paraffin: Nectin-1/PVRL1 Antibody (OTI6F8) [NBP2-01198]

Immunohistochemistry-Paraffin: PVRL1 Antibody (6F8) [NBP2-01198] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PVRL1 mouse monoclonal antibody.
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Applications for Nectin-1/PVRL1 Antibody (OTI6F8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.54 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nectin-1

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq].

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Entrez Gene IDs

5818 (Human)

Gene Symbol

NECTIN1

Additional Nectin-1 Products

Product Documents for Nectin-1/PVRL1 Antibody (OTI6F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nectin-1/PVRL1 Antibody (OTI6F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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