Nectin-1/PVRL1 Antibody (R1.302) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54643CL1
Clone R1.302 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # R1.302
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NIH/3T3 cells transfected with human CD111
Specificity
The mouse monoclonal antibody R1.302 recognizes CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Nectin-1/PVRL1 Antibody (R1.302) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Nectin-1
Long Name
Poliovirus Receptor Related 1
Alternate Names
CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1
Gene Symbol
NECTIN1
Additional Nectin-1 Products
Product Documents for Nectin-1/PVRL1 Antibody (R1.302) [CoraFluor™ 1]
Product Specific Notices for Nectin-1/PVRL1 Antibody (R1.302) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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