Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43665

Novus Biologicals, part of Bio-Techne
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NBP3-43665-100ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL15366

Concentration

1 mg/ml

View all Nectin-3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein Q9NQS3. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated]

Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Staining of rat brain shows weak to moderate positivity in neuronal processes in the CA3 area of the hippocampus.
Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Immunohistochemistry-Paraffin: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Staining of mouse brain shows weak to moderate positivity in neuronal processes in the CA3 area of the hippocampus.
Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] Western blot: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Western blot: Nectin-3/PVRL3 Antibody (CL15366) [NBP3-43665]

Analysis in human cell lines U-251MG and MCF-7 using Nectin-3/PVRL3 antibody. Corresponding NECTIN3 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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Applications for Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated]

Application
Recommended Usage

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-3

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Long Name

Poliovirus Receptor Related 3

Alternate Names

CD113, Nectin3, PPR3, PVRL3, PVRR3

Gene Symbol

NECTIN3

Additional Nectin-3 Products

Product Documents for Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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