Nectin-4/PVRL4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20014
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Predicted:
Bovine (92%), Canine (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nectin-4/PVRL4. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in (An M, Lee P, Choi S et al)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nectin-4/PVRL4 Antibody
![Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP2-20014]](https://resources.bio-techne.com/images/products/PVRL4-Antibody-Immunohistochemistry-Paraffin-NBP2-20014-img0002.jpg "Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP2-20014]")
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP2-20014]
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP2-20014] - MCF7 xenograft, using PVRL4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] -
Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] -HaCaT whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] -
Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin-4/PVRL4 antibody [N1C1] (NBP2-20014) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Nectin-4/PVRL4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nectin-4
Long Name
Poliovirus Receptor Related 4
Alternate Names
LNIR, Nectin4, PRR4, PVRL4
Gene Symbol
NECTIN4
UniProt
Additional Nectin-4 Products
Product Documents for Nectin-4/PVRL4 Antibody
Product Specific Notices for Nectin-4/PVRL4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.