NEDD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27197

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-27197
NBP2-27197SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Equine, Primate

Predicted:

Monkey (100%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all NEDD4 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 950-1000 of human NEDD4 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 94% homologous in Zebrafish, and 88% homologous in Chicken and Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEDD4 Antibody

Western Blot: NEDD4 Antibody [NBP2-27197]

Western Blot: NEDD4 Antibody [NBP2-27197]

Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for NEDD4 Antibody

Application
Recommended Usage

Western Blot

4-6ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD4

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. NEDD4 contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/kDFR, independently of its ubiquitin-ligase activity. NEDD4 acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence, NEDD4 acts as down regulator of Notch-1 gene expression.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Gene Symbol

NEDD4

UniProt

P46934 (Human)

Additional NEDD4 Products

Product Documents for NEDD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NEDD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.