NEDD8 Antibody (5B8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37523

Novus Biologicals, part of Bio-Techne
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NBP2-37523
NBP2-37523-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B8

Format

BSA Free

Concentration

1 mg/ml

View all NEDD8 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEDD8 Antibody (5B8) - BSA Free

Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8) [NBP2-37523] - Western blot analysis using NEDD8 mouse mAb against C6 (1) and Hela (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunocytochemistry/Immunofluorescence: NEDD8 Antibody (5B8) [NBP2-37523] - Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunohistochemistry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunohistochemistry: NEDD8 Antibody (5B8) [NBP2-37523] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 mouse mAb with DAB staining.
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Applications for NEDD8 Antibody (5B8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD8

NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Gene Symbol

NEDD8

Additional NEDD8 Products

Product Documents for NEDD8 Antibody (5B8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEDD8 Antibody (5B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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