NEDD8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68758

Novus Biologicals, part of Bio-Techne
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NBP2-68758
NBP2-68758-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEDD8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEDD8 Antibody

Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody [NBP2-68758]

Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody [NBP2-68758]

Immunocytochemistry/Immunofluorescence: NEDD8 Antibody [NBP2-68758] - Staining of human cell line U-2 OS shows localization to nucleoplasm.

Applications for NEDD8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD8

NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Gene Symbol

NEDD8

Additional NEDD8 Products

Product Documents for NEDD8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEDD8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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