NEDP1/SENP8 Antibody (2E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00123228-M06

Novus Biologicals, part of Bio-Techne
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H00123228-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEDP1/SENP8 Antibodies »

Product Specifications

Immunogen

SENP8 (NP_660205, 113 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLIATLAKK

Specificity

SENP8 - SUMO/sentrin specific peptidase family member 8 (2E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NEDP1/SENP8 Antibody (2E1)

Western Blot: NEDP1/SENP8 Antibody (2E1) [H00123228-M06]

Western Blot: NEDP1/SENP8 Antibody (2E1) [H00123228-M06]

Western Blot: NEDP1/SENP8 Antibody (2E1) [H00123228-M06] - Analysis of SENP8 expression in transfected 293T cell line by SENP8 monoclonal antibody (M06), clone 2E1. Lane 1: SENP8 transfected lysatE (24.1 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: NEDP1/SENP8 Antibody (2E1) [H00123228-M06] - Detection limit for recombinant GST tagged SENP8 is 0.3 ng/ml as a capture antibody.

Applications for NEDP1/SENP8 Antibody (2E1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEDP1/SENP8

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM]

Alternate Names

DEN1, Deneddylase-1, PRSC2, SENP8

Entrez Gene IDs

123228 (Human)

Gene Symbol

SENP8

UniProt

NP_660205

Additional NEDP1/SENP8 Products

Product Documents for NEDP1/SENP8 Antibody (2E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NEDP1/SENP8 Antibody (2E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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