NEDP1/SENP8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85259

Novus Biologicals, part of Bio-Techne
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NBP1-85259
NBP1-85259-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEDP1/SENP8 Antibodies »

Product Summary for NEDP1/SENP8 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEDP1/SENP8 Antibody

Western Blot: NEDP1/SENP8 Antibody [NBP1-85259]

Western Blot: NEDP1/SENP8 Antibody [NBP1-85259]

Western Blot: NEDP1/SENP8 Antibody [NBP1-85259] - Analysis in control (vector only transfected HEK293T lysate) and SENP8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NEDP1/SENP8 Antibody [NBP1-85259]

Immunocytochemistry/ Immunofluorescence: NEDP1/SENP8 Antibody [NBP1-85259]

Immunocytochemistry/Immunofluorescence: NEDP1/SENP8 Antibody [NBP1-85259] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: NEDP1/SENP8 Antibody [NBP1-85259]

Immunohistochemistry-Paraffin: NEDP1/SENP8 Antibody [NBP1-85259]

Immunohistochemistry-Paraffin: NEDP1/SENP8 Antibody [NBP1-85259] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for NEDP1/SENP8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDP1/SENP8

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM]

Alternate Names

DEN1, Deneddylase-1, PRSC2, SENP8

Gene Symbol

SENP8

Additional NEDP1/SENP8 Products

Product Documents for NEDP1/SENP8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEDP1/SENP8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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