NEK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82883

Novus Biologicals, part of Bio-Techne
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NBP1-82883-25ul
NBP1-82883
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEK1 Antibodies »

Product Summary for NEK1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEK1 Antibody

Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883]

Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883]

Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Analysis in human testis and skin tissues using antibody. Corresponding NEK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883]

Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883]

Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody NBP1-82883 (A) shows similar protein distribution across tissues to independent antibody NBP1-82884 (B).
Immunocytochemistry/ Immunofluorescence: NEK1 Antibody [NBP1-82883]

Immunocytochemistry/ Immunofluorescence: NEK1 Antibody [NBP1-82883]

Immunocytochemistry/Immunofluorescence: NEK1 Antibody [NBP1-82883] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.
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Applications for NEK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK1

Belonging to the NEK Ser/Thr protein kinase family, NEK1 plays an important role in the cell by responding to DNA damage. Molecular functions for NEK1 include protein serine/threonine kinase activity, protein tyrosine kinase activity, protein binding and ATP binding. More specifically, NEK1 is involved in cell cycle regulation, and the protein encoded by this gene is a serine/threonine kinase which is found in a centrosomal complex with FEZ1. Additionally, NEK1 also appears to possess tyrosine kinase activity and is involved in the control of meiosis and cilium assembly. NEK1 is known to interact with FEZ1, FEZ2, KIF3A, TSC2 and MRE11A. Common diseases for NEK1 include adenocarcinoma, cancer, short rib-polydactyly syndrome II and epithelial neoplasia.

Alternate Names

DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2

Gene Symbol

NEK1

Additional NEK1 Products

Product Documents for NEK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NEK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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