NEK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36533
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by antibody maps to a region between residue 975 to 1025 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEK1 Antibody
Immunoprecipitation: NEK1 Antibody [NBP2-36533]
Immunoprecipitation: NEK1 Antibody [NBP2-36533] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody NBP2-36533 used for IP at 6 ug per reaction. NEK1 was also immunoprecipitated by rabbit anti-NEK1 antibody NBP2-36534. For blotting immunoprecipitated NEK1, NBP2-36534 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for NEK1 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NEK1
Alternate Names
DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2
Gene Symbol
NEK1
UniProt
Additional NEK1 Products
Product Documents for NEK1 Antibody
Product Specific Notices for NEK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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