NEK2 Antibody (1B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41207

Novus Biologicals, part of Bio-Techne
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NBP1-41207
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEK2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide Immunogen sequence information is commercially sensitive

Localization

Nuclear. Localizes at the centromere. Isoform 1 has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end missing in isoform 2 which is predominantly cytoplasmic.

Specificity

This antibody will recognize human NEK2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK2 Antibody (1B6)

NEK2 Antibody (1B6)

Western Blot: NEK2 Antibody (1B6) [NBP1-41207] -

Western Blot: NEK2 Antibody (1B6) [NBP1-41207] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (NBP1-41207) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for NEK2 Antibody (1B6)

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
WB: Detects both isoforms 1 and 2 in Jurkat lysate.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK2

NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.Both isoforms are expressed in pheripheral T cells and many other transformed cell types.

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Entrez Gene IDs

4751 (Human)

Gene Symbol

NEK2

Additional NEK2 Products

Product Documents for NEK2 Antibody (1B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NEK2 Antibody (1B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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