NEK6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14415

Novus Biologicals, part of Bio-Techne
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NBP3-14415
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Monkey (100%), Orangutan (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

1 mg/ml

View all NEK6 Antibodies »

Product Specifications

Immunogen

Synthetic 14 amino acid peptide from N-Terminus of human NEK6.

Epitope

N-Terminus

Reactivity Notes

Expected reactivity based on percent sequence identity: Mouse, Rat, Hamster, Canine, Equine, Porcine (86%)

Specificity

Human NEK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for NEK6 Antibody

Immunohistochemistry: NEK6 Antibody [NBP3-14415]

Immunohistochemistry: NEK6 Antibody [NBP3-14415]

Immunohistochemistry: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of breast carcinoma
Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415]

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415]

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human testis after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415]

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415]

Immunohistochemistry-Paraffin: NEK6 Antibody [NBP3-14415] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lymph node, non-Hodgkins lymphoma, after heat-induced antigen retrieval.
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Applications for NEK6 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: NEK6

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Gene Symbol

NEK6

Additional NEK6 Products

Product Documents for NEK6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEK6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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