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NEK8 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93834

Novus Biologicals, part of Bio-Techne
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NBP2-93834-0.02ml
NBP2-93834-0.1ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 270-390 of human NEK8 (NP_835464.1). GSVRMRRAEKSVAPSNTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK8 Antibody - Azide and BSA Free

Western Blot: NEK8 AntibodyAzide and BSA Free [NBP2-93834]

Western Blot: NEK8 AntibodyAzide and BSA Free [NBP2-93834]

Western Blot: NEK8 Antibody [NBP2-93834] - Western blot analysis of extracts of various cell lines, using NEK8 antibody (NBP2-93834) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for NEK8 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK8

NEK8 (NIMA-Related Kinase 8) belongs to the protein kinase superfamily. Mutations in NEK8 have been linked to nephronophthisis type 9. NEK8 is required for renal tubular integrity. NEK8 is known to have interactions with ABL1, FYN, ACVR1, BICD2 and CSN2.

Alternate Names

EC 2.7.11, EC 2.7.11.1, JCKNIMA-related kinase 12a, MGC138445, NEK12A, nephrocystin 9, Never in mitosis A-related kinase 8, NIMA (never in mitosis gene a)- related kinase 8, Nima-related protein kinase 12a, NimA-related protein kinase 8, NPHP9NIMA-family kinase NEK8, serine/threonine-protein kinase Nek8, serine/thrionine-protein kinase NEK8

Gene Symbol

NEK8

Additional NEK8 Products

Product Documents for NEK8 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEK8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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