Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NELF-E Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human negative elongation factor polypeptide E using the numbering given in entry NP_002895.3 (GeneID 7936).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NELF-E Antibody

Western Blot: NELF-E Antibody [NBP1-05978]

Western Blot: NELF-E Antibody [NBP1-05978]

Western Blot: NELF-E Antibody [NBP1-05978] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody used for IP at 3 mcg/mg lysate. NELFE was also immunoprecipitated by rabbit anti-NELFE antibody NBP1-05979, which recognizes a downstream epitope.
Western Blot: NELF-E Antibody [NBP1-05978]

Western Blot: NELF-E Antibody [NBP1-05978]

Western Blot: NELF-E Antibody [NBP1-05978] - Detection of whole cell lysate from HeLa (15 & 50 ug), 293T (50 ug) and Jurkat (50 ug) cells, with diluted at 0.4 ug/ml.
Immunoprecipitation: NELF-E Antibody [NBP1-05978]

Immunoprecipitation: NELF-E Antibody [NBP1-05978]

Immunoprecipitation: NELF-E Antibody [NBP1-05978] - Detection of human NELFE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody A301-913A (lot A301-913A-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NELFE was also immunoprecipitated by a previous lot (lot A301-913A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for NELF-E Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NELF-E

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq]

Alternate Names

D6S45, negative elongation factor E, negative elongation factor polypeptide E, NELFE, NELF-ERDmajor histocompatibility complex gene RD, nuclear protein, RD RNA binding protein, RD RNA-binding protein, RDP, RNA-binding protein RD

Entrez Gene IDs

7936 (Human)

Gene Symbol

NELFE

UniProt

P18615 (Human)

Additional NELF-E Products

Product Documents for NELF-E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NELF-E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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