Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Neogenin Antibodies »

Product Summary for Neogenin Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1411 to 1461 of human Neogenin using the numbering given in entry NP_002490.2 (GeneID 4756).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neogenin Antibody

Western Blot: Neogenin Antibody [NBP2-32272]

Western Blot: Neogenin Antibody [NBP2-32272]

Western Blot: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Neogenin Antibody [NBP2-32272]

Immunoprecipitation: Neogenin Antibody [NBP2-32272]

Immunoprecipitation: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for IP at 6 ug per reaction. Neogenin was also immunoprecipitated by rabbit anti-Neogenin antibody BL16480. For blotting immunoprecipitated Neogenin, NBP2-32272 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Neogenin Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Neogenin

Neogenin is one of several netrin-binding proteins. Neogenin functions as an Repulsive Guidance Molecule (RGM) receptor; RGM is implicated in axonal guidance and neural tube closure.

Alternate Names

NEO, NEO1, NGN

Gene Symbol

NEO1

UniProt

NP_002490.2 (Human)

Additional Neogenin Products

Product Documents for Neogenin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neogenin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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