Nephrin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18058
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody [NBP3-18058]](https://resources.bio-techne.com/images/products/Nephrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18058-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Nephrin Antibody [NBP3-18058]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to the internal portion of human Nephrin (amino acids 23-1029) [UniProt O60500].
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nephrin Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody [NBP3-18058]
Immunocytochemistry/Immunofluorescence: Nephrin Antibody [NBP3-18058] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Nephrin Antibody (NBP3-18058) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]](https://resources.bio-techne.com/images/products/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0002.jpg "Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]")
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]](https://resources.bio-techne.com/images/products/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0001.jpg "Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]")
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058]
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.Applications for Nephrin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nephrin
Alternate Names
CNF, NPHS1
Gene Symbol
NPHS1
Additional Nephrin Products
Product Documents for Nephrin Antibody - BSA Free
Product Specific Notices for Nephrin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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