Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 212504

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Neprilysin/CD10 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Met45-Pro743
Accession # P08473

Specificity

Detects human Neprilysin/CD10 in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human ECE-2 and recombinant mouse Neprilysin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Neprilysin/CD10 Antibody (212504) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody (212504) [PE/Cy5.5]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Neprilysin/CD10 Antibody (212504) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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