Nesprin-3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93890
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3). LRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nesprin-3 Antibody - Azide and BSA Free
Western Blot: Nesprin-3 AntibodyAzide and BSA Free [NBP2-93890]
Western Blot: Nesprin-3 Antibody [NBP2-93890] - Analysis of extracts of various cell lines, using Nesprin-3 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: Nesprin-3 Antibody - Azide and BSA Free [NBP2-93890]
Immunocytochemistry/Immunofluorescence: Nesprin-3 Antibody [NBP2-93890] - Immunofluorescence analysis of NIH/3T3 cells using Nesprin-3 Rabbit pAb (NBP2-93890) at dilution of 1:100. Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: Nesprin-3 Antibody - Azide and BSA Free [NBP2-93890]
Immunocytochemistry/Immunofluorescence: Nesprin-3 Antibody [NBP2-93890] - Immunofluorescence analysis of HeLa cells using Nesprin-3 Rabbit pAb (NBP2-93890) at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for Nesprin-3 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nesprin-3
Alternate Names
chromosome 14 open reading frame 49, FLJ25605, MGC75267, nesprin-3, NET53
Gene Symbol
SYNE3
Additional Nesprin-3 Products
Product Documents for Nesprin-3 Antibody - Azide and BSA Free
Product Specific Notices for Nesprin-3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov