Nestin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-265

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Canine, Mouse (Negative), Rabbit (Negative), Rat (Negative)

Cited:

Human, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Nestin Antibodies »

Product Specifications

Immunogen

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.

Localization

Cytoplasmic

Marker

Neural Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nestin Antibody

Western Blot: Nestin Antibody [NB300-265]

Western Blot: Nestin Antibody [NB300-265]

Western Blot: Nestin Antibody [NB300-265] - Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug). Lane 1: 0.5 ug/ml NB 300-265: lane 2: 2 ug/ml NB 300-265. ECL: 1 second exposure.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB300-265]

Immunocytochemistry/ Immunofluorescence: Nestin Antibody [NB300-265]

Immunocytochemistry/Immunofluorescence: Nestin Antibody [NB300-265] - The Nestin antibody was tested in NTERA2 cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Applications for Nestin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000
Application Notes
This Nestin antibody may be used in Immunocytochemistry/Immunofluorescence and Western blot where a band is seen at ~220-240 kDa. Another band may be seen ~110-115 kDa. Other applications have not been tested. Though this production has not been tested, past productions have been useful for Immunohistochemistry and Immunoprecipitation. The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

UniProt

P48681

Additional Nestin Products

Product Documents for Nestin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nestin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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