Netrin-1 Antibody (JE38-87)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32643

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32643
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE38-87

Concentration

1 mg/ml

View all Netrin-1 Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Netrin-1 aa 505-604 / 604. (Uniprot: O95631)

Localization

Secreted > extracellular space > extracellular matrix.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Netrin-1 Antibody (JE38-87)

Netrin-1 Antibody (JE38-87)

Western Blot: Netrin-1 Antibody (JE38-87) [NBP3-32643]

Western Blot: Netrin-1 Antibody (JE38-87) [NBP3-32643] - Western blot analysis of Netrin-1 on different lysates with Rabbit anti-Netrin-1 antibody (NBP3-32643) at 1/1,000 dilution.

Lane 1: Human small intestine tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse heart tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32643) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Netrin-1 Antibody (JE38-87)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Netrin-1

Semaphorins, neuropilins and netrins are among a number of molecules and their receptors that regulate the developing nervous system to guide the development of neural circuits.1 Although first identified as axon guidance cues,2,3 it is now apparent that many of these same factors are not limited to the guidance of growing axons, but have roles in a range of processes from the guidance of cell migration to the regulation of the immune response, angiogenesis, lung branching morphogenesis, nervous system regeneration, and cancer.4-9 The semaphorins make up the largest family of axon guidance cues. They are characterized by the presence of an approximately 500 amino acid N-terminal semaphorin (Sema) domain. Semaphorins function mainly as chemorepellents that direct axons away from tissues.3 Semaphorin 3A (Sema3A) has been shown to be repellent to cortical axons and to inhibit axon branching.10 The transmembrane protein semaphorin 6A has been shown to repel embryonic sympathetic axons.11 The actions of the various semaphorins are not always similar. Semaphorin 3A has been found to inhibit tumor development whereas semaphorin 6A may contribute to tumor progression.9 Neuropilins are the ligand binding moieties in the class 3 Semaphorin receptor complexes that subsequently activate signaling through associated plexins. Two types have been identified so far: Neuropilin-1 (Npn-1) and Neuropilin-2 (Npn-2) receptors. At the amino acid sequence level, Npn-1 and Npn-2 share 44% identity. Npn-1 and Npn-2 show different expression patterns in developing neurons of the central and peripheral nervous systems, and show different binding specificities for different members of he semaphorin family. Both also function as receptors for some forms of vascular endothelial growth factor (VEGF).12 Netrins are a family of laminin-related small proteins that are involved in axon guidance and eurite outgrowth. Netrin-1 has been shown to attract cortical growth cones and promote axon branching.10 Netrin-4 (first named b-netrin) was found to promote neurite elongation from olfactory bulb explants.

Alternate Names

Netrin1, NTN1

Gene Symbol

NTN1

Additional Netrin-1 Products

Product Documents for Netrin-1 Antibody (JE38-87)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Netrin-1 Antibody (JE38-87)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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