Neuregulin-1/NRG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19588

Novus Biologicals, part of Bio-Techne
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NBP2-19588
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Neuregulin-1/NRG1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Neuregulin-1/NRG1. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%).

Localization

Pro-neuregulin-1; membrane-bound isoform: Cell membrane; Single-pass type I membrane protein; Neuregulin-1: Secreted; Isoform 8: Nucleus; Isoform 9: Secreted; Isoform 10: Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neuregulin-1/NRG1 Antibody

Western Blot: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Western Blot: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Western Blot: Neuregulin-1/NRG1 Antibody [NBP2-19588] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody [C1C3] diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Immunocytochemistry/ Immunofluorescence: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Immunocytochemistry/Immunofluorescence: Neuregulin-1/NRG1 Antibody [NBP2-19588] - Neuregulin-1 antibody detects Neuregulin-1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuregulin-1 stained by Neuregulin-1 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Western Blot: Neuregulin-1/NRG1 Antibody [NBP2-19588]

Western Blot: NRG1 Antibody [NBP2-19588] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:2000.
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Applications for Neuregulin-1/NRG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:500-1:3000
Application Notes
ICC/IF Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Neuregulin-1/NRG1

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]

Alternate Names

Heregulin-1, HGL, HRG1-alpha, HRG1-beta 1, Neuregulin1, NRG1

Gene Symbol

NRG1

UniProt

Q02297

Additional Neuregulin-1/NRG1 Products

Product Documents for Neuregulin-1/NRG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neuregulin-1/NRG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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