NeuroD1 Antibody (3H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00004760-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NeuroD1 Antibodies »

Product Specifications

Immunogen

NEUROD1 (AAH09046, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFT

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27320912).

Specificity

NEUROD1 - neurogenic differentiation 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NeuroD1 Antibody (3H8)

Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01]

Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01]

Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01]

Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01]

Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01]

Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01]

Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
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Applications for NeuroD1 Antibody (3H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. Use in Immunohistochemistry reported in scientific literature (PMID: 27320912).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NeuroD1

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.

Long Name

Neurogenic Differentiation Factor 1

Alternate Names

BETA2, BHF-1, NIDDM

Entrez Gene IDs

4760 (Human)

Gene Symbol

NEUROD1

OMIM

125853 (Human)

UniProt

AAH09046 (Human)

Additional NeuroD1 Products

Product Documents for NeuroD1 Antibody (3H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NeuroD1 Antibody (3H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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