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Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72956

Novus Biologicals, part of Bio-Techne
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NBP2-72956

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.077 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free

Western Blot: Neurogenin-3 Antibody (OTI3B5)Azide and BSA Free [NBP2-72956]

Western Blot: Neurogenin-3 Antibody (OTI3B5)Azide and BSA Free [NBP2-72956]

Western Blot: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogeni
Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]

Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]

Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.
Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]

Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]

Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - Neurogenin3 Staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.

Applications for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50 - 1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neurogenin-3

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[supplied by OMIM]

Alternate Names

Atoh5, Math4B, NEUROG3, Neurogenin3, Relax

Gene Symbol

NEUROG3

Additional Neurogenin-3 Products

Product Documents for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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