Neurogranin Antibody (PSH02-96)

Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645]

Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat hippocampus tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 8 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645]

Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32645) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645]

Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Neurogranin with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32645, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).