Neurogranin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29349

Novus Biologicals, part of Bio-Techne
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NBP2-29349
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Neurogranin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KIKSGERGRKG, from the internal region of the protein sequence according to NP_006167.1.

Reactivity Notes

Expected from sequence similarity: Mouse, Canine and Bovine. This batch is not expected to cross-react with Mouse in Western blot

Specificity

Reported variants represent identical protein: NP_006167.1, NP_001119653.1 Immunogen

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Neurogranin Antibody

Western Blot: Neurogranin Antibody [NBP2-29349]

Western Blot: Neurogranin Antibody [NBP2-29349]

Western Blot: Neurogranin Antibody [NBP2-29349] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-29349]

Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-29349]

Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-29349] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Neurogranin Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

detection limit 1:8000

Western Blot

0.1-0.3ug/ml
Application Notes
Western blot:Approx 16kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6kDa according to NP_006167.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498).
IHC: Paraffin embedded Human Brain (Cortex).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Neurogranin

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. (provided by RefSeq)

Alternate Names

hng, NRGN, RC3

Gene Symbol

NRGN

UniProt

Q92686 (Mouse)

Additional Neurogranin Products

Product Documents for Neurogranin Antibody

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Certificate of Analysis

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Product Specific Notices for Neurogranin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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